Journal
TOXINS
Volume 13, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/toxins13080557
Keywords
zearalenone; selenomethionine; IPEC-J2; oxidative stress; Nrf2; Keap1 signaling
Categories
Funding
- Natural Science Foundation of Heilongjiang Province of China [LC2018007]
- National Key RD Program [2016YFD0501207]
- China Agriculture Research System of MOF and MARA [CARS-35]
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The study found that selenomethionine could partly reverse the oxidative damage and cell death caused by Zearalenone toxins, through a Nrf2/Keap1-ARE pathway.
Zearalenone (ZEN) is a kind of nonsteroidal mycotoxin that is considered a risk affecting the safety of human food and livestock feed that causes oxidative damages in mammalian cells. Selenomethionine (SeMet) was indicated to have antioxidant activity and received great interest in investigating the role of SeMet as a therapeutic agent in oxidation. Therefore, the aim of this study was to investigate the hormetic role of DL-SeMet in porcine intestinal epithelial J2 (IPEC-J2) cells against ZEN-induced oxidative stress injury. As a result of this experiment, 30 mu g/mL of ZEN was observed with significantly statistical effects in cell viability. Following the dose-dependent manner, 20 mu g/mL was chosen for the subsequent experiments. Then, further results in the current study showed that the ZENinduced oxidative stress with subsequent suppression of the expression of antioxidant stress pathway-related genes species. Moreover, SeMet reversed the oxidative damage and cell death of ZEN toxins to some extent, by a Nrf2/Keap1-ARE pathway. The finding of this experiment provided a foundation for further research on the ZEN-caused cell oxidative damage and the cure technology.
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