Journal
TOXINS
Volume 13, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/toxins13060429
Keywords
dye-decolorizing peroxidase; mycotoxin; aflatoxin B-1; zearalenone; deoxynivalenol; biotransformation
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Funding
- National Key Research and Development Program of China [2021YFC2100200]
- State Key Laboratory of Animal Nutrition Project [2004DA125184G2101]
- China Agriculture Research System of MOF
- China Agriculture Research System of MARA [CARS-41]
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This study demonstrates that BsDyP is a promising candidate for the simultaneous degradation of multiple mycotoxins in animal feed and food, with lower toxicities of its degradation products.
The co-occurrence of multiple mycotoxins, including aflatoxin B-1 (AFB(1)), zearalenone (ZEN) and deoxynivalenol (DON), widely exists in cereal-based animal feed and food. At present, most reported mycotoxins degrading enzymes target only a certain type of mycotoxins. Therefore, it is of great significance for mining enzymes involved in the simultaneous degradation of different types of mycotoxins. In this study, a dye-decolorizing peroxidase-encoding gene BsDyP from Bacillus subtilis SCK6 was cloned and expressed in Escherichia coli BL21/pG-Tf2. The purified recombinant BsDyP was capable of oxidizing various substrates, including lignin phenolic model compounds 2,6-dimethylphenol and guaiacol, the substrate 2,2 '-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), anthraquinone dye reactive blue 19 and azo dye reactive black 5, as well as Mn2+. In addition, BsDyP could efficiently degrade different types of mycotoxins, including AFB(1), ZEN and DON, in presence of Mn2+. More important, the toxicities of their corresponding enzymatic degradation products AFB(1)-diol, 15-OH-ZEN and C15H18O8 were significantly lower than AFB(1), ZEN and DON. In summary, these results proved that BsDyP was a promising candidate for the simultaneous degradation of multiple mycotoxins in animal feed and food.
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