Formulation and Antibacterial Activity Evaluation of Quaternized Aminochitosan Membrane for Wound Dressing Applications

Much attention has been paid to chitosan biopolymer for advanced wound dressing owing to its exceptional biological characteristics comprising biodegradability, biocompatibility and respectable antibacterial activity. This study intended to develop a new antibacterial membrane based on quaternized aminochitosan (QAMCS) derivative. Herein, aminochitosan (AMCS) derivative was quaternized by N-(2-Chloroethyl) dimethylamine hydrochloride with different ratios. The pre-fabricated membranes were characterized by several analysis tools. The results indicate that maximum surface potential of +42.2 mV was attained by QAMCS3 membrane compared with +33.6 mV for native AMCS membrane. Moreover, membranes displayed higher surface roughness (1.27 +/- 0.24 mu m) and higher water uptake value (237 +/- 8%) for QAMCS3 compared with 0.81 +/- 0.08 mu m and 165 +/- 6% for neat AMCS membranes. Furthermore, the antibacterial activities were evaluated against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus cereus. Superior antibacterial activities with maximum inhibition values of 80-98% were accomplished by QAMCS3 membranes compared with 57-72% for AMCS membrane. Minimum inhibition concentration (MIC) results denote that the antibacterial activities were significantly boosted with increasing of polymeric sample concentration from 25 to 250 mu g/mL. Additionally, all membranes unveiled better biocompatibility and respectable biodegradability, suggesting their possible application for advanced wound dressing.

Automated summary

A new antibacterial membrane based on quaternized aminochitosan derivative was developed, showing higher surface potential, roughness, and water uptake compared to native aminochitosan membrane. The membranes exhibited superior antibacterial activities against various bacteria, with increasing polymeric sample concentration enhancing the antibacterial effects. Additionally, all membranes demonstrated good biocompatibility and respectable biodegradability, indicating their potential application for advanced wound dressing.