4.7 Article

Nanofiber-Mache Hollow Ball Mimicking the Three-Dimensional Structure of a Cyst

Journal

POLYMERS
Volume 13, Issue 14, Pages -

Publisher

MDPI
DOI: 10.3390/polym13142273

Keywords

electrospinning; nanofiber; hollow ball; alginate; tissue engineering; 3D structure

Funding

  1. Terumo Life Science Foundation [19K09502]
  2. Japan Society for the Promotion of Science

Ask authors/readers for more resources

A novel hollow nanofiber sphere model was designed for investigating the development of intracranial epidermoid cysts, allowing observation of oriented cell migration and collagen fibril secretion in the structure.
The occasional malignant transformation of intracranial epidermoid cysts into squamous cell carcinomas remains poorly understood; the development of an in vitro cyst model is urgently needed. For this purpose, we designed a hollow nanofiber sphere, the nanofiber-mache ball. This hollow structure was fabricated by electrospinning nanofiber onto alginate hydrogel beads followed by dissolving the beads. A ball with approximately 230 mm(3) inner volume provided a fibrous geometry mimicking the topography of the extracellular matrix. Two ducts located on opposite sides provided a route to exchange nutrients and waste. This resulted in a concentration gradient that induced oriented migration, in which seeded cells adhered randomly to the inner surface, formed a highly oriented structure, and then secreted a dense web of collagen fibrils. Circumferentially aligned fibers on the internal interface between the duct and hollow ball inhibited cells from migrating out of the interior, similar to a fish bottle trap. This structure helped to form an adepithelial layer on the inner surface. The novel nanofiber-mache technique, using a millimeter-sized hollow fibrous scaffold, is excellently suited to investigating cyst physiology.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available