Effect of Relative Humidity on the Electrospinning Performance of Regenerated Silk Solution

Recently, the electrospun silk web has been intensively studied in terms of its biomedical applications, including tissue engineering scaffolds, due to its good biocompatibility, cytocompatibility, and biodegradability. In this study, the effect of relative humidity (RH) conditions on the morphology of electrospun silk fiber and the electrospinning production rate of silk solution was examined. In addition, the effect of RH on the molecular conformation of electrospun silk web was examined using Fourier transform infrared (FTIR) spectroscopy. As RH was increased, the maximum electrospinning rate of silk solution and fiber diameter of the resultant electrospun silk web were decreased. When RH was increased to 60%, some beads were observed, which showed that the electrospinnability of silk formic acid solution deteriorated with an increase in RH. The FTIR results showed that electrospun silk web was partially beta-sheet crystallized and RH did not affect the molecular conformation of silk.

Automated summary

The study found that as the relative humidity increased, the electrospinning rate of silk solution and the fiber diameter of the silk web decreased, leading to the formation of beads. FTIR results showed that the electrospun silk web was partially beta-sheet crystallized, and the relative humidity did not affect the molecular conformation of silk.