4.8 Article

NIR light-responsive bacteria with live bio-glue coatings for precise colonization in the gut

Journal

CELL REPORTS
Volume 36, Issue 11, Pages -

Publisher

CELL PRESS
DOI: 10.1016/j.celrep.2021.109690

Keywords

-

Categories

Funding

  1. National Key Research and Development Program of China [2019YFA0906500, 2017YFA0205104]
  2. National Natural Science Foundation of China [31971300, 817719709, 51873150]
  3. Tianjin Natural Science Foundation [19JCYBJC28800]
  4. Young Elite Scientists Sponsorship Program by Tianjin
  5. Key Project of Tianjin Foundational Research (JingJinJi) Program, China [19JCZDJC64100]

Ask authors/readers for more resources

Recombinant bacterial colonization is essential in disease prevention, alleviation, and treatment, with the success of application hinges on efficient spatiotemporal colonization of host gut. The use of engineered upconversion microgels and light-responsive bacteria in this study demonstrated controlled and effective colonization of EcN, which successfully alleviated DSS-induced colitis in mice, potentially advancing the clinical application of engineered microbial therapeutics.
Recombinant bacterial colonization plays an indispensable role in disease prevention, alleviation, and treatment. Successful application mainly depends on whether bacteria can efficiently spatiotemporally colonize the host gut. However, a primary limitation of existing methods is the lack of precise spatiotemporal regulation, resulting in uncontrolled methods that are less effective. Herein, we design upconversion microgels (UCMs) to convert near-infrared light (NIR) into blue light to activate recombinant light-responsive bacteria (Lresb) in vivo, where autocrine ``functional cellular glues'' made of adhesive proteins assist Lresb inefficiently colonizing the gut. The programmable engineering platform is further developed for the controlled and effective colonization of Escherichia coli Nissle 1917 (EcN) in the gut. The colonizing bacteria effectively alleviate DSS-induced colitis in mice. We anticipate that this approach could facilitate the clinical application of engineered microbial therapeutics to accurately and effectively regulate host health.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.8
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available