A reference map of sphingolipids in murine tissues

Sphingolipids (SPs) have both a structural role in the cell membranes and a signaling function that regulates many cellular processes. The enormous structural diversity and low abundance of many SPs pose a challenge for their identification and quantification. Recent advances in lipidomics, in particular liquid chromatography (LC) coupled with mass spectrometry (MS), provide methods to detect and quantify many low-abundant SP species reliably. Here we use LC-MS to compile a murine sphingolipid atlas,'' containing the qualitative and quantitative distribution of 114 SPs in 21 tissues of a widely utilized wild-type laboratory mouse strain (C57BL/6). We report tissue-specific SP fingerprints, as well as sex-specific differences in the same tissue. This is a comprehensive, quantitative sphingolipidomic map of mammalian tissues collected in a systematic fashion. It will complement other tissue compendia for interrogation into the role of SP in mammalian health and disease.

Automated summary

This study utilized liquid chromatography coupled with mass spectrometry to compile a murine sphingolipid atlas, revealing the distribution of 114 SP species in different tissues and discovering tissue-specific and sex-specific differences. This comprehensive research provides insights into the role of sphingolipids in mammalian health and disease.