Journal
ADVANCED HEALTHCARE MATERIALS
Volume 10, Issue 12, Pages -Publisher
WILEY
DOI: 10.1002/adhm.202100238
Keywords
3D‐ printed sacrificial templates; anisotropic microchannels; cellular infiltration; nanofiber aerogels; patterned macrochannels
Funding
- National Institute of General Medical Science (NIGMS) at the NIH [R01GM123081, R01GM138552, R01GM134036, P30GM127200]
- National Institute of Dental and Craniofacial Research (NIDCR) at the NIH [1R21DE027516]
- National Cancer Institute (NCI) at the NIH [R00CA201603]
- National Science Foundation [NSF-CBET1936105, NE LB606]
- University of Nebraska Medical Center
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A new method for fabricating 3D PCL/gelatin nanofiber aerogels with patterned macrochannels and anisotropic microchannels is described. These aerogels show increased cellular infiltration and promote the formation of a microvascular network. Following subcutaneous implantation in rats, the aerogels exhibit enhanced cellular infiltration rates and host tissue integration.
A new approach is described for fabricating 3D poly(epsilon-caprolactone) (PCL)/gelatin (1:1) nanofiber aerogels with patterned macrochannels and anisotropic microchannels by freeze-casting with 3D-printed sacrificial templates. Single layer or multiple layers of macrochannels are formed through an inverse replica of 3D-printed templates. Aligned microchannels formed by partially anisotropic freezing act as interconnected pores between templated macrochannels. The resulting macro-/microchannels within nanofiber aerogels significantly increase preosteoblast infiltration in vitro. The conjugation of vascular endothelial growth factor (VEGF)-mimicking QK peptide to PCL/gelatin/gelatin methacryloyl (1:0.5:0.5) nanofiber aerogels with patterned macrochannels promotes the formation of a microvascular network of seeded human microvascular endothelial cells. Moreover, nanofiber aerogels with patterned macrochannels and anisotropic microchannels show significantly enhanced cellular infiltration rates and host tissue integration compared to aerogels without macrochannels following subcutaneous implantation in rats. Taken together, this novel class of nanofiber aerogels holds great potential in biomedical applications including tissue repair and regeneration, wound healing, and 3D tissue/disease modeling.
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