Aerobic exercise intensity does not affect the anabolic signaling following resistance exercise in endurance athletes

This study examined whether intensity of endurance stimulus within a concurrent training paradigm influenced the phosphorylation of signaling proteins associated with the mTOR and AMPK networks. Eight male cyclists completed (1) resistance exercise (RES), 6x8 squats at 80% 1-RM; (2) resistance exercise and moderate intensity cycling of 40 min at 65% V.O-2peak, (RES+MIC); (3) resistance exercise and high intensity interval cycling of 40 min with 6 alternating 3 min intervals of 85 and 45% V.O-2peak (RES+HIIC), in a cross-over design. Muscle biopsies were collected at rest and 3 h post-RES. There was a main effect of condition for mTOR(S2448) (p=0.043), with a greater response in the RES+MIC relative to RES condition (p=0.033). There was a main effect of condition for AMPK alpha 2(T172) (p=0.041), with a greater response in RES+MIC, relative to both RES+HIIC (p=0.026) and RES (p=0.046). There were no other condition effects for the remaining protein kinases assessed (p>0.05). These data do not support a molecular interference effect in cyclists under controlled conditions. There was no intensity-dependent regulation of AMPK, nor differential activation of anabolism with the manipulation of endurance exercise intensity.

Automated summary

The study did not find support for a molecular interference effect in cyclists under controlled conditions. There was no intensity-dependent regulation of AMPK, nor differential activation of anabolism with the manipulation of endurance exercise intensity.