Journal
GENES & DEVELOPMENT
Volume 30, Issue 11, Pages 1339-1356Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.278275.116
Keywords
genome stability; homologous recombination; Sgs1-Top3; Smc complexes; Smc5/6
Categories
Funding
- Spanish Ministry of Economy and Competitivity [BFU2015-71308-P, BFU2013-50245-EXP]
- intramural programme of Medical Research Council UK
- Wellcome Trust [100955]
- MRC [MC_U120074328] Funding Source: UKRI
- Medical Research Council [MC_U120074328] Funding Source: researchfish
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The RecQ helicase Sgs1 plays critical roles during DNA repair by homologous recombination, from end resection to Holliday junction (HJ) dissolution. Sgs1 has both pro- and anti-recombinogenic roles, and therefore its activity must be tightly regulated. However, the controls involved in recruitment and activation of Sgs1 at damaged sites are unknown. Here we show a two-step role for Smc5/6 in recruiting and activating Sgs1 through SUMOylation. First, auto-SUMOylation of Smc5/6 subunits leads to recruitment of Sgs1 as part of the STR (Sgs1-Top3-Rmi1) complex, mediated by two SUMO-interacting motifs (SIMs) on Sgs1 that specifically recognize SUMOylated Smc5/6. Second, Smc5/6-dependent SUMOylation of Sgs1 and Top3 is required for the efficient function of STR. Sgs1 mutants impaired in recognition of SUMOylated Smc5/6 (sgs1-SIM Delta) or SUMO-dead alleles (sgs1-KR) exhibit unprocessed HJs at damaged replication forks, increased crossover frequencies during double-strand break repair, and severe impairment in DNA end resection. Smc5/6 is a key regulator of Sgs1's recombination functions.
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