Enhancement and analysis of Anthracene degradation by Tween 80 in LMS-HOBt

This study examines the specific effect of Tween 80 on the conversion of anthracene (ANT) in laccase medium system regarding surfactant chemical changes and mechanism. The conversion rate and degradation products of ANT were investigated in different concentrations of Tween 80 solution. Between Tween 80 concentration 0-40 critical micelle concentrations (CMC), the kinetic parameter-k (h(-1)) and corresponding half-life T-1/2 decreased with increasing concentration. When Tween 80 was above 20 CMC the laccase-medium system converted>95% of ANT to anthraquinone within 12 h. During the entire enzymatic reaction, the laccase activity in the system increased with increasing Tween 80 concentration. Combined with GC/MS analysis of the product, it was speculated that hydrogens belonging to the ether-oxygen bond and carbon-carbon double bond alpha -CH of Tween 80, were removed by the laccase-media system, promoting its degradation. Additionally, enhanced activity caused by oxygen free radicals (ROS) such as RO center dot and ROO center dot, continuously oxidized Tween 80, which in turn produced free radicals while converting ANT. This study provides new theoretical support toward the application of surfactants in the elimination of polycyclic aromatic hydrocarbons.

Automated summary

This study demonstrates that Tween 80 can enhance the conversion of anthracene in laccase medium system, with over 95% conversion to anthraquinone achievable within 12 hours when Tween 80 concentration exceeds a certain threshold. Additionally, the presence of Tween 80 increases laccase activity in the system, facilitating the degradation of anthracene.