Journal
SCIENTIFIC REPORTS
Volume 11, Issue 1, Pages -Publisher
NATURE RESEARCH
DOI: 10.1038/s41598-021-90115-8
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Funding
- Chuck Fowler Foundation, Cleveland, OH
- Abraham J. & Phyllis Katz Foundation, Atlanta, GA
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Co-culturing human iTregs with MSCs enhances the stability of FOXP3 expression and suppressive function, with a key mechanism being MSC mt transfer via tunneling nanotubules. Mitochondrial metabolic function drives MSC mt transfer to proliferating iTregs, stabilizing FOXP3 expression and promoting suppressive function in inflammatory conditions.
The key obstacle to clinical application of human inducible regulatory T cells (iTreg) as an adoptive cell therapy in autoimmune disorders is loss of FOXP3 expression in an inflammatory milieu. Here we report human iTreg co-cultured with bone marrow-derived mesenchymal stromal cells (MSCs) during short-term ex vivo expansion enhances the stability of iTreg FOXP3 expression and suppressive function in vitro and in vivo, and further that a key mechanism of action is MSC mitochondrial (mt) transfer via tunneling nanotubules (TNT). MSC mt transfer is driven by mitochondrial metabolic function (CD39/CD73 signaling) in proliferating iTreg and promotes iTreg expression of FOXP3 stabilizing factors BACH2 and SENP3. These results elucidate cellular and molecular mechanisms underlying human MSC mt transfer to proliferating cells. MSC mt transfer stabilizes FOXP3 expression in iTregs, thereby enhancing and sustaining their suppressive function in inflammatory conditions in vitro and in vivo.
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