Journal
NUTRIENTS
Volume 13, Issue 9, Pages -Publisher
MDPI
DOI: 10.3390/nu13093242
Keywords
enzyme-assisted extraction; non-extractable polyphenols; pressurized liquid extraction; proanthocyanidins; sweet cherry pomace
Categories
Funding
- Comunidad of Madrid (Spain)
- FSE Program
- FEDER Program (AVANSECAL-II-CM) [S2018/BAA-4393]
- Comunidad of Madrid
- University of Alcala (SOSBIO) [CM/JIN/2019-033]
- Spanish Ministry of Science and Innovation [PID2020-114891RB-100]
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The study investigated the release of non-extractable polyphenols (NEPs) from sweet cherry pomace using pressurized liquid extraction (PLE) combined with enzyme-assisted extraction (EAE). The PLE-EAE extracts exhibited higher total phenolic content and antioxidant capacity compared to traditional extraction methods, with higher molecular weight NEPs being obtained through this innovative approach.
Sweet cherry generates large amounts of by-products within which pomace can be a source of bioactive phenolic compounds. Commonly, phenolic compounds have been obtained by conventional extraction methodologies. However, a significant fraction, called non-extractable polyphenols (NEPs), stays held in the conventional extraction residues. Therefore, in the present work, the release of NEPs from cherry pomace using pressurized liquid extraction (PLE) combined with enzyme-assisted extraction (EAE) using Promod(TM) enzyme is investigated for the first time. In order to study the influence of temperature, time, and pH on the NEPs extraction, a response surface methodology was carried out. PLE-EAE extracts displayed higher TPC (75 +/- 8 mg GAE/100 g sample) as well as, PA content, and antioxidant capacity than the extracts obtained by PLE (with a TPC value of 14 +/- 1 mg GAE/100 g sample) under the same extraction conditions, and those obtained by conventional methods (TPC of 8.30 +/- 0.05 mg GAE/100 g sample). Thus, PLE-EAE treatment was more selective and sustainable to release NEPs from sweet cherry pomace compared with PLE without EAE treatment. Besides, size-exclusion chromatography profiles showed that PLE-EAE allowed obtaining NEPs with higher molecular weight (>8000 Da) than PLE alone.
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