4.6 Article

Effects of Different Chenopodium formosanum Parts on Antioxidant Capacity and Optimal Extraction Analysis by Taguchi Method

Journal

MATERIALS
Volume 14, Issue 16, Pages -

Publisher

MDPI
DOI: 10.3390/ma14164679

Keywords

antioxidant; Chenopodium formosanum; leaf; extract; design of experiment; Taguchi method

Funding

  1. National United University
  2. Ministry of Science and Technology of Taiwan [MOST 108-2218-E-239-003, MOST 110-2622-E-239-003]

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Chenopodium formosanum (CF), a native plant in Taiwan, is rich in nutrients and antioxidants. The seeds and leaves have higher radical scavenging ability than stems and roots, making them suitable as a source of antioxidants and aiding in waste reduction.
Chenopodium formosanum (CF), rich in nutrients and antioxidants, is a native plant in Taiwan. During the harvest, the seeds are collected, while the roots, stems, and leaves remain on the field as agricultural waste. In this study, di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (DPPH) radical scavenging ability and 2,2 '-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability experiments of seeds, leaves, stems, and roots were designed using the Taguchi method (TM) under three conditions: Ethanol concentration (0-100%), temperature (25-65 degrees C), and extraction time (30-150 min). The result demonstrates that seeds and leaves have higher radical scavenging ability than stems and roots. Many studies focused on CF seeds. Therefore, this study selected CF leaves and optimized DPPH, ABTS, total phenol content (TPC), total flavonoid content (TFC), and reducing power (RP) through TM, showing that the predicted value of the leaf is close to the actual value. The optimized results of CF leaves were DPPH 85.22%, ABTS 46.51%, TPC 116.54 mu g GAE/mL, TFC 143.46 mu g QE/mL, and RP 23.29 mu g VCE (vitamin C equivalent)/mL. The DPPH and ABTS of CF leaves were second only to the results of CF seeds. It can be seen that CF leaves have the potential as a source of antioxidants and help in waste reduction.

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