Journal
GENE
Volume 575, Issue 2, Pages 331-338Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.gene.2015.09.010
Keywords
Boleophthalmus pectinirostris; HSP70; Testis; Immunohistochemistty; Injury
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Funding
- National Natural Science Foundation of China [31272642, 41276151, 31572603]
- Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, K.C. Wong Magna Fund in Ningbo University, Scientific and Technical Project of Zhejiang Province [2012C12907-8]
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Heat shock protein 70 (HSP70) is molecular chaperone that is important for reproductive biological processes. In this study, a full length HSP70 from the mudskipper (Boleophthalmus pectinirostris) was characterized. It was found to contain: a 108 bp 5'-untranslated region, a 208 bp 3'-untranslated region, and a 1953 bp open reading frame, which encodes a protein of 650 amino acids with a theoretical molecular weight of 71.1 kDa and an isoelectric point of 5.17. RT-PCR analysis revealed that HSP70 was ubiquitously expressed in all major tissues with differential expression levels. This suggests that HSP70 has vital and conserved biological functions. HSP70 was localized mainly in the cytoplasm of germinal cells, indicating an important role of this protein during spermatogenesis. In response to heat stress, the testes presented abnormal morphology in connective tissues, in which HSP70 immunoreactivity was not observed. HSP70 mRNA expression in the gill, liver, and testes was significantly increased, which suggests that HSP70 plays an important role in protection against heat stress. (C) 2015 Elsevier B.V. All rights reserved.
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