Enzymatic Hydroxylations of sp3-Carbons

Enzymatic hydroxylation of activated and nonactivated sp(3)-carbons attracts keen interest from the chemistry community as it is one of the most challenging tasks in organic synthesis. Nature provides a vast number of enzymes with an enormous catalytic versatility to fulfill this task. Given that those very different enzymes have a distinct specificity in substrate scope, selectivity, activity, stability, and catalytic cycle, it is interesting to outline similarities and differences. In this Review, we intend to delineate which enzymes possess considerable advantages within specific issues. Heterologous production, crystal structure availability, enzyme engineering potential, and substrate promiscuity are essential factors for the applicability of these biocatalysts.

Automated summary

The enzymatic hydroxylation of activated and nonactivated sp(3)-carbons is challenging in organic synthesis, but nature provides a variety of enzymes with catalytic versatility to tackle this task. Different enzymes have distinct specificity in substrate scope, selectivity, activity, stability, and catalytic cycle, and factors such as heterologous production, crystal structure availability, enzyme engineering potential, and substrate promiscuity play crucial roles in the applicability of these biocatalysts.