Journal
NATURE COMMUNICATIONS
Volume 12, Issue 1, Pages -Publisher
NATURE RESEARCH
DOI: 10.1038/s41467-021-23934-y
Keywords
-
Categories
Funding
- Czech Science Foundation [18-10035S, 21-04132S]
- Agence Nationale de la Recherche [ANR-16-CE11-0032-04]
- Institut national du Cancer [INCa PLBio 2016-161]
- Ligue Nationale Contre le Cancer
- MEYS [LM2015062, CZ.02.1.01/0.0/0.0/16_013/0001775]
- OPPK [CZ.2.16/3.1.00/21547]
- [RVO68378050]
- [RVO68378050-KAV-NPUI]
Ask authors/readers for more resources
The study revealed that TSSC4 is a component of U5 snRNP and plays a critical role in promoting tri-snRNP formation, providing new insights into the biogenesis and recycling process of spliceosomal U5 snRNP.
U5 snRNP is a complex particle essential for RNA splicing. U5 snRNPs undergo intricate biogenesis that ensures that only a fully mature particle assembles into a splicing competent U4/U6 center dot U5 tri-snRNP and enters the splicing reaction. During splicing, U5 snRNP is substantially rearranged and leaves as a U5/PRPF19 post-splicing particle, which requires re-generation before the next round of splicing. Here, we show that a previously uncharacterized protein TSSC4 is a component of U5 snRNP that promotes tri-snRNP formation. We provide evidence that TSSC4 associates with U5 snRNP chaperones, U5 snRNP and the U5/PRPF19 particle. Specifically, TSSC4 interacts with U5-specific proteins PRPF8, EFTUD2 and SNRNP200. We also identified TSSC4 domains critical for the interaction with U5 snRNP and the PRPF19 complex, as well as for TSSC4 function in tri-snRNP assembly. TSSC4 emerges as a specific chaperone that acts in U5 snRNP de novo biogenesis as well as post-splicing recycling. The correct assembly and recycling of the multicomponent spliceosome remains largely elusive. Here, the authors show that a previously uncharacterized protein TSSC4 associates with de novo formed spliceosomal U5 snRNP as well as with a post-splicing U5-PRPF19 particle, and that TSSC4 is important for assembly of the splicing competent tri-snRNP.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available