Comparative genomic analysis reveals metabolic flexibility of Woesearchaeota

The archaeal phylum Woesearchaeota, within the DPANN superphylum, includes phylogenetically diverse microorganisms that inhabit various environments. Their biology is poorly understood due to the lack of cultured isolates. Here, we analyze datasets of Woesearchaeota 16S rRNA gene sequences and metagenome-assembled genomes to infer global distribution patterns, ecological preferences and metabolic capabilities. Phylogenomic analyses indicate that the phylum can be classified into ten subgroups, termed A-J. While a symbiotic lifestyle is predicted for most, some members of subgroup J might be host-independent. The genomes of several Woesearchaeota, including subgroup J, encode putative [FeFe] hydrogenases (known to be important for fermentation in other organisms), suggesting that these archaea might be anaerobic fermentative heterotrophs.

Automated summary

The archaeal phylum Woesearchaeota, belonging to the DPANN superphylum, consists of phylogenetically diverse microorganisms that exhibit a symbiotic lifestyle, with some exceptions in subgroup J. Genomic analyses suggest that these archaea may be anaerobic fermentative heterotrophs, based on the presence of putative [FeFe] hydrogenases in their genomes.