Journal
EXPERIMENTAL AND THERAPEUTIC MEDICINE
Volume 22, Issue 3, Pages -Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/etm.2021.10375
Keywords
microRNA-374a-5p; NCK adaptor protein 1; non-small cell lung cancer; tumor suppressor
Categories
Funding
- Pre-clinical Transformation Research of panel, a Multigene Molecular Marker for Early Diagnosis of Lung Adenocarcinoma ('Office-School Consultation Fund-Science and Technology Innovation' Project of Hebei Science and Technology Department-Hebei Medical Uni [2020TXZH04]
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The study indicates that miR-374a-5p acts as a tumor suppressor in NSCLC by targeting NCK1, leading to inhibition of cell proliferation and migration. Lower levels of miR-374a-5p were observed in NSCLC cells compared to normal cells, highlighting the importance of the miR-374a-5p/NCK1 axis in NSCLC.
Emerging studies have indicated that microRNAs (miRNAs/miRs) are involved in regulating non-small cell lung cancer (NSCLC)-associated processes. The present study aimed to evaluate the biological roles of miR-374a-5p in NSCLC. Using reverse transcription-quantitative PCR, the expression levels of miR-374a-5p were determined in NSCLC cells and a normal cell line. Functional experiments were performed to investigate the functions of miR-374a-5p in NSCLC. A luciferase activity reporter assay and rescue experiments were performed to validate NCK adaptor protein 1 (NCK1) as a functional target of miR-374a-5p. It was demonstrated that miR-374a-5p levels were decreased in NSCLC cell lines compared with those in a normal cell line. Furthermore, overexpression of miR-374a-5p inhibited NSCLC cell proliferation and migration in vitro. Of note, NCK1 overexpression reversed the effects of miR-375a-5p on NSCLC cell proliferation and migration. The present results confirmed the tumor suppressor role of miR-374a-5p via targeting NCK1 in NSCLC, indicating the importance of the miR-374a-5p/NCK1 axis in NSCLC.
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