The association between bacterobilia and the risk of postoperative complications following pancreaticoduodenectomy

Background: Pre-operative biliary bacterial colonisation (bacterobilia) is considered a risk factor for infectious complications after pancreaticoduodenectomy (PD). This study aimed to investigate the role of the PD biliary microbiome grown in the development of post-PD complications. Methods: In a retrospective study of 162 consecutive patients undergoing PD (2008-2018), intra-operative bile cultures were analyzed and sensitivities compared to pre-anesthetic antibiotics and thirty-day post-surgery complications. Results: Bacterobilia was present in 136 patients (84%). Most bile cultures grew bacteria resistant to pre-operative antibiotics (n = 112, 82%). Patients with bacterobilia had significantly higher rates of major complication than patients without (P = 0.017), as well as higher rates of surgical-site infections (SSI) (P = 0.010). Patients with negative bile cultures (n = 26) had significantly lower rates of major compli-cation and SSI than those growing sensitive (n = 24) or non-sensitive (n = 112) bacteria (major compli-cation P = 0.029 and SSI P = 0.011). Conclusion: Positive bile cultures were associated with a higher incidence of major complications and SSI. Patients with sterile bile cultures had the lowest risk of post-operative complications and efforts to reduce rates of bacterobilia, such as limitation of biliary instrumentation, should be considered. Sensi-tivity to antibiotics had no effect upon the rate of post-operative complications, but this may reflect low cohort numbers.

Automated summary

Pre-operative biliary bacterial colonisation is associated with infectious complications after pancreaticoduodenectomy. Patients with positive bile cultures have higher rates of major complications and surgical-site infections. Patients with sterile bile cultures have lower risk of complications.