4.6 Article

Genotype of Immunologically Hot or Cold Tumors Determines the Antitumor Immune Response and Efficacy by Fully Virulent Retargeted oHSV

Journal

VIRUSES-BASEL
Volume 13, Issue 9, Pages -

Publisher

MDPI
DOI: 10.3390/v13091747

Keywords

oncolytic virus; oncolytic herpes simplex virus; retargeting; HER2; tumor genotype; vaccination; immunotherapy; immune checkpoint inhibitors

Categories

Funding

  1. European Research Council ADG [340060]
  2. Department of Experimental Diagnostic and Specialty Medicine through the Pallotti legacy
  3. European Research Council (ERC) [340060] Funding Source: European Research Council (ERC)

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The non-attenuated HER2-retargeted oHSV named R-337 showed efficacy against the immunologically hot CT26-HER2 tumor, providing protection and long-term adaptive vaccination effects. The immune protection was based on orchestrating changes in the tumor immune profile, reversing immunosuppression and promoting antitumor responses. The study highlights the importance of characterizing tumor immune markers for more precise application of oncolytic herpesviruses.
We report on the efficacy of the non-attenuated HER2-retargeted oHSV named R-337 against the immunologically hot CT26-HER2 tumor, and an insight into the basis of the immune protection. Preliminarily, we conducted an RNA immune profiling and immune cell content characterization of CT26-HER2 tumor in comparison to the immunologically cold LLC1-HER2 tumor. CT26-HER2 tumor was implanted into HER2-transgenic BALB/c mice. Hallmarks of R-337 effects were the protection from primary tumor, long-term adaptive vaccination directed to both HER2 and CT26-wt cell neoantigens. The latter effect differentiated R-337 from OncoVEXGM-CSF. As to the basis of the immune protection, R-337 orchestrated several changes to the tumor immune profile, which cumulatively reversed the immunosuppression typical of this tumor (graphical abstract). Thus, Ido1 (inhibitor of T cell anticancer immunity) levels and T regulatory cell infiltration were decreased; Cd40 and Cd27 co-immunostimulatory markers were increased; the IFN gamma cascade was activated. Of note was the dampening of IFN-I response, which we attribute to the fact that R-337 is fully equipped with genes that contrast the host innate response. The IFN-I shut-down likely favored viral replication and the expression of the mIL-12 payload, which, in turn, boosted the antitumor response. The results call for a characterization of tumor immune markers to employ oncolytic herpesviruses more precisely.

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