Journal
VIRUS RESEARCH
Volume 305, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.virusres.2021.198578
Keywords
Delivery of CRISPR/Cas9; Recombinant snakehead rhabdovirus (SHRV); BHK-21 cells; IRF9 gene knockout
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Funding
- National Research Foundation ofKorea (NRF) - Korea government (Ministry of Science and ICT) [NRF-2020R1A2C4001511]
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The study evaluated the potential use of a piscine RNA virus SHRV as a delivery system for CRISPR/Cas9 in mammalian cells. It successfully knocked out the target gene of mammalian cells using IRF9 gene-targeting rSHRV, with the knockout efficiency possibly regulated by increasing temperature. Further optimization research is needed to enhance editing efficiency, but this study represents the first report on the possible applicability of a piscine RNA virus for gene editing in mammalian cells.
As there is no risk of viral genome integration into host chromosome, cytoplasmic RNA viruses can be a safer vehicle to deliver CRISPR/Cas system. Snakehead rhabdovirus (SHRV) is a piscine RNA virus belonging to the family Rhabdoviridae, and, in the present study, we evaluated the availability of SHRV as a tool for CRISPR/Cas9 delivery in mammalian cells. SHRV was grown well in baby hamster kidney (BHK-21) cells at 28 degrees C, and the replication ability was greatly reduced by temperature up-shift to 37 degrees C. We rescued a recombinant SHRV that harboring not only the interferon regulatory factor 9 (IRF9) gene-targeting single-guide RNA (sgRNA) but also Cas9 gene in the genome using the reverse genetic technology. The IRF9 gene of BHK-21 cells was knocked-out by the infection with the IRF9 gene-targeting rSHRV. Moreover, the rSHRVs were sharply disappeared in the cells by elevating temperature to 37 degrees C, suggesting the possible regulation of knockout efficiency before virus infection-caused cell damage. Although further optimization researches are needed to enhance the editing efficiency using the recombinant SHRV, to our knowledge, this is the first report on the possible applicability of piscine RNA virus for the gene editing in mammalian cells.
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