Journal
VIRUS RESEARCH
Volume 302, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.virusres.2021.198481
Keywords
Vibrio phage; Genome; Sequence analysis
Categories
Funding
- Key Project of Beijing University of Chemical Technology [XK1803-06]
- National Key Research and Development Program of China [2018YFA0903000, 2020YFC2005405, 2020YFA0712100, 2020YFC0840805]
- Funds for First-class Discipline Construction [XK1805]
- Inner Mongolia Key Research and Development Program [2019ZD006]
- National Natural Science Foundation of China [81672001, 81621005]
- NSFCMFST project (China-Mongolia) [31961143024]
- Fundamental Research Funds for Central Universities [BUCTRC201917, BUCTZY2022]
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Bacterial infections have a negative impact on both animal husbandry industry and medicine, and the increasing bacterial drug resistance worsens this impact. Phages present a promising alternative to combating drug-resistant bacteria, and the newly isolated phage vB_ValP_IME234 shows potential as a biocontrol agent against Vibrio alginolyticus strains.
Bacterial infections have a negative impact on both animal husbandry industry and medicine, and increasing bacterial drug resistance exacerbates this adverse impact. Phages show promise as an alternative to drugs against drug-resistant bacteria. In this study, a novel virulent bacteriophage (phage) vB_ValP_IME234 against Vibrio alginolyticus and Vibrio parahaemolyticus was isolated from freshwater in Beijing, China. Phage vB_ValP_IME234 had an isometric head (59 nm in diameter) and a short tail (10 nm long), belonging to Podoviridae family. Its complete genome is liner double-stranded DNA (dsDNA) with a GC content of 41.6% while encoding 61 putative proteins. Three transfer RNA (tRNA) and no lysogenic gene was detected. vB_ValP_IME234 had a polyvalent infectivity, a burst of 390 PFU/cell, and is stable under different temperatures (4 degrees C to 50 degrees C) and pH (6.0 to 10.0) values. Host range test showed that vB_ValP_IME234 has the ability to infect seven strains of Vibrio in total. Phylogenetic analyses based on terminase and capsid suggested that this phage had a close relationship with Vibrio phages. These results indicate that vB_ValP_IME234 could be used as a potential biocontrol agent against V. alginolyticus strains.
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