Journal
VETERINARY PATHOLOGY
Volume 58, Issue 6, Pages 1158-1171Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/03009858211030541
Keywords
mutant mice; muscle; phenotyping; muscular diseases; neuropathic; neurogenic; myogenic; mutation
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The development of mouse models that replicate the genetic and pathological features of human disease is crucial for preclinical research, as they allow for meaningful pharmacokinetic, safety, and efficacy studies. Histopathology is important in identifying specific phenotypic traits that may not be detected by other physiological or behavioral screening tests. Its inclusion in phenotype screening protocols can help uncover novel genetic myopathies that may be clinically silent or missed by traditional screening methods.
The development of mouse models that replicate the genetic and pathological features of human disease is important in preclinical research because these types of models enable the completion of meaningful pharmacokinetic, safety, and efficacy studies. Numerous relevant mouse models of human disease have been discovered in high-throughput screening programs, but there are important specific phenotypes revealed by histopathology that are not reliably detected by any other physiological or behavioral screening tests. As part of comprehensive phenotypic analyses of over 4000 knockout (KO) mice, histopathology identified 12 lines of KO mice with lesions indicative of an autosomal recessive myopathy. This report includes a brief summary of histological and other findings in these 12 lines. Notably, the inverted screen test detected muscle weakness in only 4 of these 12 lines (Scyl1, Plpp7, Chkb, and Asnsd1), all 4 of which have been previously recognized and published. In contrast, 6 of 8 KO lines showing negative or inconclusive findings on the inverted screen test (Plppr2, Pnpla7, Tenm1, Srpk3, Sidt2, Yif1b, Mrs2, and Pnpla2) had not been previously identified as having myopathies. These findings support the need to include histopathology in phenotype screening protocols in order to identify novel genetic myopathies that are not clinically evident or not detected by the inverted screen test.
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