4.5 Article

Anthelmintic activity of aminoalcohol and diamine derivatives against the gastrointestinal nematode Teladorsagia circumcincta

Journal

VETERINARY PARASITOLOGY
Volume 296, Issue -, Pages -

Publisher

ELSEVIER
DOI: 10.1016/j.vetpar.2021.109496

Keywords

Teladorsagia circumcincta; Anthelmintic; Aminoalcohol; Diamine; In vitro assays

Funding

  1. RICET contract EC Programs [E07D401988BR]
  2. MINECO: RETOS [AGL2016-79813-C2-1R/2R]
  3. Junta de Castilla y Leon (JCyL)
  4. FEDER, UE [LE020P17]
  5. Junta de Castilla y Leon Fellowship Scheme for Doctoral Training Program
  6. European Social Found (ESF)'s Fellowship Scheme for Doctoral Training Program
  7. Spanish Ramon y Cajal Programme (Ministerio de Economia y Competitividad) [RYC-2015-18368]
  8. [FPU16/03536]
  9. [JCYL-USAL35B]
  10. [LE082-18]
  11. [LE051-18]

Ask authors/readers for more resources

The study evaluated the efficacy of various derivatives against Gastrointestinal nematodes, with diamine 30 showing the best results in preventing egg hatching and diamine 34 showing the highest efficacy in inhibiting larval migration.
Gastrointestinal nematodes (GIN) infections are a serious problem in livestock production due to the great economic losses they cause. Their control is increasingly difficult because of the rapid development of drug resistance and the limited number of available drugs. Therefore, this study evaluated 18 aminoalcohol and 16 diamine derivatives against eggs, first and third stage larvae from a susceptible and a resistant isolate of Teladorsagia circumcincta collected from sheep. The effectiveness of the in vitro anthelmintic activity of the compounds was evaluated using three different procedures: Egg Hatch Test (EHT), Larval Mortality Test (LMT) and Larval Migration Inhibition Test (LMIT). Those compounds with activities higher than 90 % in the initial screening at 50 tiM were selected to determine their half maximal effective concentration (EC50). In parallel, cytotoxicity assays were conducted on Caco2 and HepG2 cell lines to calculate Selectivity Indexes (SI) for each compound. The diamine 30 presented the best results in preventing egg hatching, displaying the lowest EC50 value (1.01 +/- 0.04 mu M) of all compounds tested and the highest SI (21.21 vs. Caco-2 cells). For the LMIT, the diamine 34 showed the highest efficacy, with EC50 values of 2.67 +/- 0.08 and 3.02 +/- 0.09 mu M on the susceptible and resistant isolate of the parasite, respectively.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.5
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available