Journal
ULTRASONICS SONOCHEMISTRY
Volume 74, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.ultsonch.2021.105554
Keywords
Emulsion; Physical stability; Myofibrillar protein; High-intensity ultrasound; Low ionic strength
Categories
Funding
- Major Science and Technology Projects of Heilongjiang Province [2020ZX07B02, 2019ZX07B03]
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The study showed that using high-intensity ultrasound (HIU) can effectively improve the physical stability of myofibrillar protein (MP) emulsions in low-salt media by promoting protein adsorption and molecular rearrangement at the oil-water interface to achieve droplet stabilization.
The specific molecular behavior of myofibrillar proteins (MPs) in low-salt media limits the development of muscle protein-based emulsions. This study aimed to evaluate the potential of high-intensity ultrasound (HIU; 150, 300, 450, and 600 W) to improve the physical stability of MP emulsion at low ionic strength and decipher the underlying mechanism. According to the physical stability analysis, HIU pretreatment, especially at 450 W power, significantly improved the physical stability of MP emulsions, as evidenced by the reduced particle size, enhanced inter-droplet interactions, and increased uniformity of the droplet size distribution (p < 0.05). The results of interfacial protein composition, Fourier transform infrared spectroscopy analysis, and microscopic morphology observation of the aqueous MP suspension suggested that HIU induced the depolymerization of filamentous myosin polymers and inhibited the subsequent self-assembly behavior. These effects may facilitate protein adsorption and molecular rearrangement at the oil-water interface, forming a complete interfacial layer and, thus, droplet stabilization. Confocal laser scanning microscopy observations further confirmed these results. In conclusion, these findings provide direct evidence for the role of HIU in improving the physical stability of MP emulsions at low ionic strength.
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