Journal
TRANSGENIC RESEARCH
Volume 30, Issue 6, Pages 727-737Publisher
SPRINGER
DOI: 10.1007/s11248-021-00280-9
Keywords
AgGlpF gene; Transgenic soybean; Salt tolerance
Categories
Funding
- JAAS [KYJF2021JQ001]
- National Natural Science Foundation of China [31501327]
- Youth Scientific Research Foundation of Jilin [20150520123JH]
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This study successfully transferred the salt-tolerant fungus AgGlpF gene into soybean using genetic engineering, resulting in transgenic soybean varieties with significantly improved salt stress tolerance.
Salt stress is an important abiotic factor that causes severe losses in soybean yield and quality. Therefore, breeding salt-tolerant soybean germplasm resources via genetic engineering has gained importance. Aspergillus glaucus, a halophilic fungus that exhibits significant tolerance to salt, carries the gene AgGlpF. In this study, we used the soybean cotyledonary node transformation method to transfer the AgGlpF gene into the genome of the soybean variety Williams 82 to generate salt-tolerant transgenic soybean varieties. The results of PCR, Southern blot, ddPCR, and RT-PCR indicated that AgGlpF was successfully integrated into the soybean genome and stably expressed. When subjected to salt stress conditions via treatment with 250 mM NaCl for 3 d, the transgenic soybean plants showed significant tolerance compared with wild-type plants, which exhibited withering symptoms and leaf abscission after 9 d. The results of this study indicated that the transfer of AgGlpF into the genome of soybean plants produced transgenic soybean with significantly improved salt stress tolerance.
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