Journal
TISSUE & CELL
Volume 71, Issue -, Pages -Publisher
CHURCHILL LIVINGSTONE
DOI: 10.1016/j.tice.2021.101506
Keywords
Advanced-platelet-rich fibrin extract; Adipose-derived stem cells; Differentiation; Paracrine
Categories
Funding
- National Natural Science Foundation of China [81760346, 81860341]
- Scientific Research & Technology Development Program of Guangxi [Guikegong 1598012-1]
- Scientific Research & Technology Development Program of Nanning City [20173021-2, 20183037-1]
- Innovation Project of Guangxi Graduate Education [YCBZ2018041]
- science and technology support program of Jiangxi Province [20151BBG70080]
- Self-financing Research Project of Guangxi Province Health and Family Planning Commission [Z20180679, Z20190139]
- Youth Science Innovation and Entrepreneurship Talent Training Project of Nanning [RC20180201]
- International Communication of Guangxi Medical University Graduate Education
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This study aimed to identify the optimal concentrations of A-PRFe to promote adipogenic and osteogenic differentiation of human adipose-derived stem cells (ASCs). Results showed that A-PRFe significantly stimulated ASC proliferation and enhanced adipogenic/osteogenic differentiation and paracrine function at higher concentrations.
Advanced platelet-rich fibrin (A-PRF) is an autogenous biological material obtained from peripheral blood. APRF extract (A-PRFe) contains a high concentration of various cytokines that are increasingly appreciated for their roles in improving stem cell repairing function during tissue regeneration. However, the optimal A-PRFe concentration to stimulate stem cells is unknown. This study aimed to identify the optimal concentrations of A-PRFe to promote adipogenic and osteogenic differentiation of human adipose-derived stem cells (ASCs). We produced A-PRFe from A-PRF clots by centrifuging fresh peripheral blood samples and isolated and identified ASCs using surface CD markers and multilineage differentiation potential. Enzyme-linked immunosorbent assay (ELISA) showed the concentrations of several cytokines, including b-FGF, PDGF-BB, and others, increased gradually, peaked on day 7 and then decreased. Cell proliferation assays showed A-PRFe significantly stimulated ASC proliferation, and proliferation significantly increased at higher A-PRFe doses. The degree of adipogenic and osteogenic differentiation increased at higher A-PRFe concentrations in the culture medium, as determined by oil red O and alizarin red staining. Reverse transcription polymerase chain reaction (RT-PCR) showed that expression levels of genes related to adipogenic/osteogenic differentiation (PPAR gamma 2, C/EBP alpha, FABP4, Adiponectin, and ALP, OPN, OCN, RUNX2), paracrine (HIF-1 alpha, VEGF, IGF-2) and immunoregulation (HSP70, IL-8) function were higher in groups with a higher concentration of A-PRFe than in lower concentration groups. This study demonstrates that A-PRFe is ideal for use in ASC applications in regenerative medicine because it improves biological functions, including proliferation, adipogenic/osteogenic differentiation, and paracrine function in a dose-dependent manner.
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