Journal
TALANTA
Volume 229, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2021.122275
Keywords
Electrochemical sensors; Multiparametric platform; Stress metabolites monitoring; Microfluidic technology
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Funding
- European Union's Horizon 2020 research and innovation program [760921]
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This paper presents the design and development of a multiparametric (bio) sensing platform for real-time monitoring of cell metabolites, highlighting its easy manufacturing process, easy scalability, modularity, versatility, and cost-effectiveness. The developed platform allows for multiparametric analysis of key factors indicating potential cell damage during cell culture. The proposal of a sensors platform based on thick-film coupled to microfluidic technology for accurate analysis of secreting stress metabolites during cell/tissues culture is also discussed.
There is a growing need for real-time monitoring of metabolic products that could reflect cell damages over extended periods. In this paper, we report the design and development of an original multiparametric (bio) sensing platform that is tailored for the real-time monitoring of cell metabolites derived from cell cultures. Most attractive features of our developed electrochemical (bio)sensing platform are its easy manufacturing process, that enables seamless scale-up, modular and versatile approach, and low cost. In addition, the developed platform allows a multiparametric analysis instead of single-analyte analysis. Here we provide an overview of the sensors-based analysis of four main factors that can indicate a possible cell deterioration problem during cell-culture: pH, hydrogen peroxide, nitric oxide/nitrite and lactate. Herein, we are proposing a sensors platform based on thick-film coupled to microfluidic technology that can be integrated into any microfluidic system using Luer-lock connectors. This platform allows obtaining an accurate analysis of the secreting stress metabolites during cell/tissues culture.
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