A novel fluorescent strategy based on double modifications of metal organic framework material CAU-10-NH2 for low background and high sensitivity determination of H2S

Hydrogen sulfide is typical metabolic marker and environmental pollutant which is worthwhile to determine. Herein, a low background and high sensitivity fluorescent strategy based on double modifications of metal organic framework material CAU-10-NH2 is proposed for the determination of hydrogen sulfide. Firstly, a functional monomer 3,5-diaminobenzoic acid is employed to modify on the CAU-10-NH2, the product CAU-10-NH-dAba has strong fluorescent performance at 412 nm under an excitation wavelength of 320 nm. Subsequently, it is further modified by the azide group to form CAU-10-NH-dAba-N-3. This azidation inhibits the fluorescent signal. However, in the presence of hydrogen sulfide, the azide group is specifically reduced to amidogen, and results in the recovery of the fluorescence. The CAU-10-NH-DABA-N-3 was characterized by solid state NMR, XPS, fluorescence, IR, XRD, SEM and specific surface area. After the optimization of pH value, temperature and interaction time, the detection results of hydrogen sulfide demonstrate the linear range of this strategy is from 20 to 140 nM with a detection limit of 1.51 nM, which is significantly better than that of the CAU-10-NH2 merely modified by 3,5-dinitrobenzoic acid. Meanwhile, the satisfactory assay results of hydrogen sulfide in serum sample and Pearl river water suggest a potential application prospect of this strategy in clinical diagnosis and environment monitoring.

Automated summary

A novel fluorescent strategy based on double modifications of metal organic framework material was proposed for the determination of hydrogen sulfide, demonstrating low background and high sensitivity. After optimization, the strategy showed a wide linear range and low detection limit, with satisfactory assay results in serum samples and Pearl river water.