Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 343, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2021.130104
Keywords
Porphyrin; Dodecyl ammonium bromide; Peroxidase-like catalyst; Glucose; Hydrogen peroxide; Colorimetric biosensor
Funding
- National Key R&D Program of China [2020YFC1712700]
- National Natural Science Foundation of China [31972164, 21776321, 32001789, 21665022, 21776259]
- Guizhou Provincial Science and Technology Department [QKHJC [2017] 1186, QKHZC [2019] 2816, QKHPTRC [2020] 5009]
- Talented Researcher Program from Guizhou Provincial Department of Education [QJHKYZ [2018] 073]
- Tongren Science and Technology Bureau [TSKY20193]
- Talented Youth Cultivation Program from the Fundamental Research Funds for the Central Universities
- South-Central University for Nationalities [CZP20007]
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A sensitive and novel colorimetric biosensor was developed for detecting glucose and hydrogen peroxide, with high recovery rates in human serum and saliva samples, providing an effective biosensing platform for biomedical diagnosis.
Peroxidase is a popular amplifier of biosensor signals, but its application is limited by chemical instability and expensive purification. We developed a sensitive, novel colorimetric biosensor to detect glucose and hydrogen peroxide (H2O2) using Zinc tetrakis (4-pyridinyl) porphyrin (ZnTPyP)-dodecyl trimethyl ammonium bromide (DTAB) nanoparticles (NPs) as peroxidase-like catalysts. ZnTPyP-DTAB NPs can oxidize colorless 3, 3', 5, 5'tetramethylbenzidine to a blue product (652 nm) in the presence of H2O2 via electron transport between porphyrin and bromine. This peroxidation mechanism was further verified using quantum chemical calculations. The detection limits of the colorimetric ZnTPyP-DTAB NP sensor were 0.15 and 0.5 mu mol L-1 for glucose and H2O2, respectively, and the linear range was 3-70 and 8-50 mu mol L-1, respectively. Recovery ratios of the biosensor in spiked human serum and saliva samples were 100 % +/- 10 %. This simple method is robust, color stable, and reliable, thus providing an effective colorimetric biosensing platform for biomedical diagnosis.
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