Journal
SENSORS
Volume 21, Issue 14, Pages -Publisher
MDPI
DOI: 10.3390/s21144902
Keywords
ketone body; electrochemical sensor; cyclic voltammetry; non-invasive detection; point of care technology
Funding
- Ministry of Environment [1485017693, ARQ201901136003]
- Chung-Ang University
- Korea Environmental Industry & Technology Institute (KEITI) [ARQ201901136003] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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This report presents an enzyme deposited Au electrode for electrochemical measurement of acetylacetic acid in urine, demonstrating good performance using immobilized dehydrogenase and nucleotide as sensing materials in clinical samples. The proposed electrode shows promising potential as a platform for on-site monitoring of biochemical parameters such as urine ketone.
In this report, we present an enzyme deposited Au electrode for an electrochemical measurement of acetylacetic acid (AcAc) in urine. The electrode has an immobilized layer of a mixture of D-beta-hydroxybutyrate dehydrogenase (HBDH) and nicotinamide adenine dinucleotide (NADH) as sensing material to investigate its electroanalytical properties by means of cyclic voltammetry (CV). The modified electrodes are used for the detection of AcAc and present a linear current increase when the AcAc concentration increases. The electrode presents a limit of detection (LOD) of 6.25 mg/dL in the range of 6.25-100 mg/dL for investigation of clinical relevance. Finally, the electrode was evaluated using 20 patient samples. The measured results of urine ketone by the developed electrode were compared with the clinical results from a commercial kit, and the analysis showed good agreement. The proposed electrode was demonstrated to be a very promising platform as a miniaturized electrochemical analyzer for point-of-care monitoring of the critical biochemical parameters such as urine ketone.
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