Journal
REPRODUCTIVE SCIENCES
Volume 28, Issue 12, Pages 3529-3539Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s43032-021-00632-0
Keywords
Preeclampsia; Endothelial nitric oxide synthase; Gene polymorphisms; Haplotypes
Categories
Funding
- Ministry of Education, Science and Technological Development of Republic of Serbia [175075]
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-786 T/C and VNTR 4b/a eNOS gene polymorphisms are associated with the risk of preeclampsia in Serbian patients. Homozygotes for VNTR polymorphism have a higher risk of developing preeclampsia.
The aim of the present study was to analyze the distribution of genotypes and haplotypes of functional eNOS gene polymorphisms in the promoter (-786 T/C), intron 4 (VNTR4b/a) and exon 7 (894 G/T), in Serbian population of pregnant women, and establish a possible association between these polymorphisms and preeclampsia development. DNA was isolated from venous blood samples of 50 heathy pregnant women and 50 preeclampsia patients. Polymerase Chain Reaction/Restriction Fragment Length Polymorphism (PCR/RFLP) technique, with appropriate sets of primers and specific restriction enzymes, was used to determine polymorphisms in eNOS gene. Statistical analysis was done using the SPSS and HAPLOVIEW software packages. eNOS -786 T/C polymorphism was significantly associated with preeclampsia (P = 0.006). Homozygotes for the VNTR polymorphism had also an elevated risk of developing preeclampsia (OR=7.68, 95%CI (0.89-65.98)), especially the mild (OR=9.33, 95%CI (0.98-88.57)) and late form (OR=8.52, 95%CI (0.90-80.58)). The 894 G/T polymorphism was not associated with preeclampsia. G-C-b and T-4a-T haplotypes were more frequent in preeclampsia, though without reaching statistical significance. -786 T/C and VNTR 4b/a eNOS gene polymorphisms were associated with preeclampsia risk in Serbian patients.
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