4.4 Article

Extracellular vesicles of bovine small follicular fluid promote ovarian cortical stromal cell proliferation and steroidogenesis

Journal

REPRODUCTION IN DOMESTIC ANIMALS
Volume 56, Issue 11, Pages 1425-1434

Publisher

WILEY
DOI: 10.1111/rda.14007

Keywords

bovine; cell proliferation; follicular fluid EVs; ovarian cortical stromal cells; steroid hormones

Funding

  1. national major special project 'High-yield and high-quality transgenic beef cattle breeding' [2016ZX08007-002]
  2. Shaanxi Province's key research and development plan 'Dairy Cow Embryo Breeding Innovation [2020NY-019]

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The research found that 100μg/ml of extracellular vesicles can significantly promote the proliferation and hormone synthesis of bovine ovarian cortical stromal cells, which is of great significance for elucidating the cell-to-cell communication mechanisms in follicular fluid and ovarian development.
The aim of this study was to investigate the effects of extracellular vesicles (EVs) on the proliferation and steroid hormone synthesis of bovine ovarian cortical stromal cells in vitro. The release and uptake of EVs are the new mechanisms of cell-to-cell communication. Using reverse transcriptase polymerase chain reaction, enzyme-linked immunosorbent assay, TUNEL and other experiments, we found that EVs in bovine follicular fluid can promote the proliferation and synthesis of androstenedione and progesterone in ovarian cortical stromal cells. Moreover, 100 mu g/ml EVs caused the most significant effect. We conclude that EVs at 100 mu g/ml can significantly promote the proliferation and synthesis of androstenedione and progesterone in ovarian cortical stromal cells. This research is of great significance for further elucidating the regulatory role of follicular fluid EVs in follicular development and atresia and for research on the interaction of ovarian stromal cells, granulosa cells and oocytes.

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