High-throughput sequencing reveals differential expression of miRNAs in yak and cattleyak epididymis

Cattleyaks (CY) are interspecific hybrids between cattle (Bos taurus) and yak (Bos gruniens, YK) exhibiting the same prominent adaptability and higher performances than YK. MiRNAs have played an important role in the acquisition and maintenance of male fertility in reproduction, where deletion of Dicer in mice germ cells results in infertility. According to a body of evidence, the function of miRNA in the male reproductive system extends from the testis into the epididymis and, as such, regulates gene expression and contributes to regional gene expression variations. Using RNA sequencing on biological replicates, we described differentially expressed miRNAs profiles for tissue from epididymis of YK and CY. In the present study, High-throughput sequencing analysis showed that 55 differentially expressed (DE) miRNAs were identified in the epididymis of YK and CY. Among these, 43 DE miRNAs were upregulated while the remaining 12 DE miRNAs were downregulated between epididymis of YK and CY. Our results showed that the top most important DE miRNAs, bta-miR-449c, bta-miR-539, bta-miR-136, bta-miR-504, bta-miR-31 and bta-miR-222 were found to be involved in the reproductive system of CY. In addition, some targeted genes, Clusterins (CLU), Retinoic Acid Receptor a (RARa) and Hydroxy acyl glutathione Hydrolase (HAGH) and HSPH1 targeted by bta-miR-2411-3p and bta-miR-1298 were involved in the sperm motility, sperm morphology and post-testicular sperm maturation. Furthermore, GO and KEGG analyses were performed to classify the functions of target genes for DE miRNAs. In addition, RT-qPCR validation of the DE miRNAs and its targeted genes revealed that putative miRNAs are involved in the male CY infertility by altering the gene expression. Present findings may not only increase our understanding of the molecular mechanisms regulated by the miRNAs in epididymis, but also provide a valuable information to understand the male infertility mechanism of CY.

Automated summary

The study revealed that the male infertility in CY involves multiple miRNAs, which may affect testis and epididymis function by regulating gene expression.