Enhancing hydrolysis and bio-methane generation of extruded lignocellulosic wood waste using microbial pre-treatment

Extruded waste wood was subjected to microbial pre-treatment using two bacterial strains, Petronet Alfa and Petronet Omega. In phase one, the effects of strain type and dose, and incubation temperature on hydrolysis were investigated through measuring the amount of dissolved glucose content after hydrolysis. In phase two, biomethane potential (BMP) assays were conducted for microbially pre-treated wood waste under the optimum conditions obtained in phase one. Results showed that the effect of strain dose, temperature, and aeration on released glucose were statistically significant at p-value of 0.05 level. At the optimum conditions of using Petronet Omega at a dose of 5.0 g/L and 37 degrees C, the microbial pre-treatment could increase the amount of dissolved glucose by as much as 205%. The findings of BMP assays showed improvement in biogas production as a result of the substrate pre-treatment by Petronet Alfa and Petronet Omega where a biomethane yield of 54.4 and 49.1 mL CH4/g VS were obtained, respectively, which correspond to 88.9 and 70.2% increase compared with the control. Among four kinetic models tested, the modified Gompertz model demonstrated the best performance and showed that the lag phase was significantly reduced due to microbial pre-treatment of substrate. (C) 2021 Elsevier Ltd. All rights reserved.

Automated summary

Microbial pre-treatment of extruded waste wood using Petronet Alfa and Petronet Omega significantly increased the amount of dissolved glucose and biomethane production, with optimal conditions identified. Results showed a 205% increase in dissolved glucose content and 88.9% and 70.2% increase in biomethane yield with Petronet Alfa and Petronet Omega treatment, respectively.