Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 118, Issue 23, Pages -Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2104598118
Keywords
single-molecule calorimeter; plasmonic imaging; optical tweezer; complete thermodynamic profile
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Funding
- National Natural Science Foundation of China [21773117, 21874070, 21925403, 21904062]
- Fundamental Research Funds for the Central Universities [14380234]
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A nanoparticle-based technique has been developed to probe the energetic contributions of single-molecule binding events, introducing a single-molecule calorimeter that uncovers the complexity of molecular interactions and provides a comprehensive thermodynamic profile.
The precise measurement of thermodynamic and kinetic properties for biomolecules provides the detailed information for a multitude of applications in biochemistry, biosensing, and health care. However, sensitivity in characterizing the thermodynamic binding affinity down to a single molecule, such as the Gibbs free energy (Gb), enthalpy (Hb), and entropy (Sb), has not materialized. Here, we develop a nanoparticle-based technique to probe the energetic contributions of single-molecule binding events, which introduces a focused laser of optical tweezer to an optical path of plasmonic imaging to accumulate and monitor the transient local heating. This single-molecule calorimeter uncovers the complex nature of molecular interactions and binding characterizations, which can be employed to identify the thermodynamic equilibrium state and determine the energetic components and complete thermodynamic profile of the free energy landscape. This sensing platform promises a breakthrough in measuring thermal effect at the single-molecule level and provides a thorough description of biomolecular specific interactions.
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