Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 118, Issue 35, Pages -Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2025647118
Keywords
pericentromeric RNA; senescence-associated secretory phenotype; CTCF; small extracellular vesicles; senescence
Categories
Funding
- Japan Science and Technology Agency (JST)-Precursory Research for Embryonic Science and Technology (PRESTO) [JPMJPR17H7]
- JST-Moonshot R and D [JPMJPS2022]
- Japan Agency of Medical Research and Development-Advanced Research and Development Programs for Medical Innovation (PRIME) [19gm6110023h0001]
- Japan Society for the Promotion of Science (JSPS) [19H03507, 18K15254, 20K16344]
- Princess Takamatsu Cancer Research Fund
- Mitsubishi Foundation
- Takeda Science Foundation
- Research Fellowships for Young Scientists from JSPS [19J00796]
- Grants-in-Aid for Scientific Research [20K16344, 19H03507, 19J00796, 18K15254] Funding Source: KAKEN
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Cellular senescence leads to changes in chromatin organization and increased expression of inflammatory genes, affecting various age-related pathologies through SASP. Pericentromeric ncRNA impairs CTCF DNA binding, activating transcription of SASP-like inflammatory genes and promoting malignant transformation. The transfer of pericentromeric ncRNA through small extracellular vesicles contributes to a cell-autonomous and non-cell-autonomous SASP-like inflammatory response.
Cellular senescence causes a dramatic alteration of chromatin organization and changes the gene expression profile of proinflammatory factors, thereby contributing to various age-related pathologies through the senescence-associated secretory phenotype (SASP). Chromatin organization and global gene expression are maintained by the CCCTC-binding factor (CTCF); however, the molecular mechanism underlying CTCF regulation and its association with SASP gene expression remains unclear. We discovered that noncoding RNA (ncRNA) derived from normally silenced pericentromeric repetitive sequences directly impairs the DNA binding of CTCF. This CTCF disturbance increases the accessibility of chromatin and activates the transcription of SASP-like inflammatory genes, promoting malignant transformation. Notably, pericentromeric ncRNA was transferred into surrounding cells via small extracellular vesicles acting as a tumorigenic SASP factor. Because CTCF blocks the expression of pericentromeric ncRNA in young cells, the down-regulation of CTCF during cellular senescence triggers the up-regulation of this ncRNA and SASP-related inflammatory gene expression. In this study, we show that pericentromeric ncRNA provokes chromosomal alteration by inhibiting CTCF, leading to a SASP-like inflammatory response in a cell-autonomous and non-cell-autonomous manner and thus may contribute to
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