Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 118, Issue 39, Pages -Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2101730118
Keywords
root; suberin; endodermis; ABA; CIF
Categories
Funding
- Sandoz Family Monique De Meuron philanthropic foundation's program for academic promotion
- Swiss National Science Foundation [31003A_179159, PCEGP3_187007, 310030_188672, 31003A-170127]
- Max-Planck-Gesellschaft
- Alexander von Humboldt foundation
- Swiss National Science Foundation (SNF) [310030_188672, PCEGP3_187007, 31003A_179159, 31003A_170127] Funding Source: Swiss National Science Foundation (SNF)
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Suberin, a hydrophobic biopolymer, forms protective barriers in roots against stress. In endodermal cells, suberin deposition is regulated by a set of MYB transcription factors, controlled by developmental and environmental signals, impacting nutrient homeostasis.
Suberin is a hydrophobic biopolymer that can be deposited at the periphery of cells, forming protective barriers against biotic and abiotic stress. In roots, suberin forms lamellae at the periphery of endodermal cells where it plays crucial roles in the control of water and mineral transport. Suberin formation is highly regulated by developmental and environmental cues. However, the mechanisms controlling its spatiotemporal regulation are poorly understood. Here, we show that endodermal suberin is regulated independently by developmental and exogenous signals to fine-tune suberin deposition in roots. We found a set of four MYB transcription factors (MYB41, MYB53, MYB92, and MYB93), each of which is individually regulated by these two signals and is sufficient to promote endodermal suberin. Mutation of these four transcription factors simultaneously through genome editing leads to a dramatic reduction in suberin formation in response to both developmental and environmental signals. Most suberin mutants analyzed at physiological levels are also affected in another endodermal barrier made of lignin (Casparian strips) through a compensatory mechanism. Through the functional analysis of these four MYBs, we generated plants allowing unbiased investigation of endodermal suberin function, without accounting for confounding effects due to Casparian strip defects, and were able to unravel specific roles of suberin in nutrient homeostasis.
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