Grapevine TPS (trehalose-6-phosphate synthase) family genes are differentially regulated during development, upon sugar treatment and drought stress

Trehalose-6-phosphate synthase (TPS) performs the first step in the biosynthetic pathway of trehalose-6phosphate and trehalose. These two molecules play key roles in the control of carbon allocation and of stress responses in plants. We investigated the organization of the TPS gene family and its developmental and environmental expression regulation in grapevine, a major horticultural crop. We identified three novel genes in the family, and assessed the expression of the 11 family members in tissues and developmental phases. Two potentially biosynthetic TPS isoforms belonging to Class I were preferentially expressed in leaf (VvTPS1_A) and in fruit (VvTPS1_B) respectively. Sucrose treatment induced expression of VvTPS1_B, but not of VvTPS1_A, and a progressive decrease of sucrose concentration. Expression of a few Class II genes was affected by sucrose treatment. Application of osmotic stress by withdrawing irrigation also induced a decrease in sucrose and an increase of glucose content, and down-regulation of the VvTPS1_A gene. We discuss the possible role of these potential biosynthetic TPS genes. Subgroups of TPS genes, including both Class I and ClassII isoforms, followed a co-expression pattern in different conditions, suggesting that Class II TPS proteins may directly or indirectly interact with TPS biosynthetic genes. Our results pave the way for clarification of the role of TPS isoforms in grapevine responses to environmental stress.

Automated summary

A study on the TPS gene family in grapevine identified three new genes and found that two potential biosynthetic TPS isoforms preferentially expressed in leaf and fruit. Expression of TPS genes in grapevine was affected by sucrose treatment and osmotic stress, with different genes showing co-expression patterns in different conditions. These findings pave the way for understanding the role of TPS isoforms in grapevine responses to environmental stress.