Journal
PLANT PHYSIOLOGY AND BIOCHEMISTRY
Volume 166, Issue -, Pages 712-722Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2021.06.041
Keywords
Phosphate; Nitrogen; OsPHR3; Pi deficiency; Nitrogen use efficiency; Transgenic
Categories
Funding
- National Program on R&D of Transgenic Plants [2016ZX08009-003-005]
- Chinese National Natural Science Foundation [31672226]
- National Key Research and Development Program of China [2016yfd0100700]
- Innovative Research Team Development Plan of the Ministry of Education [IRT1256]
- 111 Project [12009]
- Shanghai Sailing Program [19YF1443200]
- Postgraduate Research & Practice Innovation Program of Jiangsu Province [KYCX17_0592, KYCX17_0594]
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Overexpression of OsPHR3 increases nitrogen uptake and utilization efficiency in rice plants under Pi stress conditions, leading to higher total nitrogen concentrations, upregulation of nitrogen transporter genes, and improved nitrogen use efficiency, 1000-grain weight, and grain yield under different Pi conditions. This study establishes that OsPHR3 overexpression facilitates nitrogen utilization under Pi deficient conditions, potentially enhancing crop yields by coordinating nitrogen and phosphorus utilization.
Phosphorus (P) and nitrogen (N) are both essential macronutrients for maintaining plant growth and development. In rice (Oryza sativa L.), OsPHR3 is one of the four paralogs of PHR1, which acts as a central regulator of phosphate (Pi) homeostasis, as well being involved in N homeostasis. However, the functions of OsPHR3 in N utilization under different Pi conditions have yet to be fully studied. In this study, we aimed to dissect the effect of OsPHR3-overexpression on N utilization under Pi deficient regimes. Biochemical, molecular and physiological assays were performed to determine the N-influx, translocation, and accumulation in OsPHR3-overexpressing rice lines, grown under Pi-sufficient and -deficient conditions, in both hydroponic and soil systems. Furthermore, important agronomic traits of these plants were also evaluated. The overexpression of OsPHR3 increased N uptake under Pi stress regimes. Increased N uptake also elevated total N concentrations in these plants by inducing N transporter genes expression. Furthermore, overexpression of OsPHR3 increased N use efficiency, 1000-grain weight and grain yield under different Pi conditions. We established new findings that OsPHR3-overexpression facilitates N utilization under Pi deficient conditions. This will help achieving higher yields by coordinating the utilization of N and P.
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