Journal
PLANT METHODS
Volume 17, Issue 1, Pages -Publisher
BMC
DOI: 10.1186/s13007-021-00763-0
Keywords
Auxin biosynthesis; Stable isotope labeling; LC-MS; Metabolic inhibitors; Pathway analysis
Categories
Funding
- Agriculture and Food Research Initiative competitive awards from the USDA National Institute of Food and Agriculture [2018-67011-28056, 2018-67013-27503, 2019-51181-30025]
- NSF Plant Genome Research Program [IOS-1238812]
- Minnesota Agricultural Experiment Station
- Gordon and Margaret Bailey Endowment for Environmental Horticulture
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The plant hormone auxin is crucial for plant growth and environmental response. Various pathways exist for the biosynthesis of IAA, the primary auxin, in different plant species. Techniques involving stable isotopes and chemical inhibitors are useful for tracing pathway utilization in vivo.
Background The plant hormone auxin plays a central role in regulation of plant growth and response to environmental stimuli. Multiple pathways have been proposed for biosynthesis of indole-3-acetic acid (IAA), the primary auxin in a number of plant species. However, utilization of these different pathways under various environmental conditions and developmental time points remains largely unknown. Results Monitoring incorporation of stable isotopes from labeled precursors into proposed intermediates provides a method to trace pathway utilization and characterize new biosynthetic routes to auxin. These techniques can be aided by addition of chemical inhibitors to target specific steps or entire pathways of auxin synthesis. Conclusions Here we describe techniques for pathway analysis in Arabidopsis thaliana seedlings using multiple stable isotope-labeled precursors and chemical inhibitors coupled with highly sensitive liquid chromatography-mass spectrometry (LC-MS) methods. These methods should prove to be useful to researchers studying routes of IAA biosynthesis in vivo in a variety of plant tissues.
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