Journal
PLANT CELL REPORTS
Volume 40, Issue 10, Pages 1907-1922Publisher
SPRINGER
DOI: 10.1007/s00299-021-02757-7
Keywords
Alfalfa; Calmodulin-like protein gene; Calcium signaling pathway; Abiotic tolerance; Transgenic tobacco
Categories
Funding
- National Natural Science Foundation of China [31770575, 31972507]
- Science and Technology Major Project of Heilongjiang Province [GA19B103]
- Excellent Youth Project of Natural Science Foundation of Heilongjiang Province [YQ2020C033]
- Graduate Innovation Fund of Harbin Normal University [HSDSSCX2020-05]
- Shanghai Agricultural Science Committee Youth Talents Development Plan [2018 (1-34)]
- Research Fund of Beijing Advanced Innovation Center for Food Nutrition and Human Health [20182018]
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MsCML46 enhances tolerance to abiotic stresses by regulating stress-related gene expression, optimizing osmolytes levels, and antioxidant enzyme activity. It binds free Ca2+ to promote signal transduction and maintain higher K+/Na+ ratio, protecting intracellular homeostasis under sodium ion toxicity.
Key message MsCML46 enhances tolerance to abiotic stresses through alleviating osmotic stress and oxidative damage by regulating the expression of stress-related genes to optimize osmolytes levels and antioxidant enzyme activity in transgenic tobacco. Abiotic stresses are major environmental factors that constraint crop productivity worldwide. Various stimuli regulate intracellular calcium levels and calcium-mediated signal transduction, and cellular responses. Ca2+ signals are perceived by different Ca2+ receptors. Calmodulin-like protein (CML) is one of the best-characterized Ca2+ sensors which shares sequence similarity with highly conserved calmodulin (CaM) ubiquitously expressed in plants. Currently, the molecular and physiological functions of CMLs are largely unknown. In this study, the MsCML46 was characterized in alfalfa (Medicago sativa cv. Zhaodong) under freezing stress. Results showed that MsCML46 was localized to the cytoplasm of Arabidopsis, and its expression was strongly elevated by cold, drought, salt, saline-alkali, and ABA treatments. Overexpressing MsCML46 in tobacco enhanced tolerance to freezing, drought, and salt stresses as evidenced by improved contents of osmotic regulatory solutes and antioxidant enzyme activity but decreased reactive oxygen species (ROS) accumulation. Furthermore, cold, drought, and salt stresses increased the expression of stress-related genes in transgenic tobacco. MsCML46 binds free Ca2+ to promote signal transduction and maintain higher K+/Na+ ratio. In this way, it protects intracellular homeostasis under sodium ion toxicity. These results suggest that MsCML46 plays a crucial role in resisting abiotic stresses and can be exploited in genetic engineering for crops.
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