Exogenous brassinosteroid and jasmonic acid improve drought tolerance in Brassica rapa L. genotypes by modulating osmolytes, antioxidants and photosynthetic system

Key message Exogenously supplied BR and JA help KS101 and KBS3 genotypes of Brassica rapa to alleviate drought stress by modifying osmolyte concentration, levels of antioxidant enzymes and photosynthetic system. Oilseed plants are susceptible to drought stress and a significant loss in yield has been reported during recent decades. Thus, it is imperative to understand the various underlying drought response mechanisms in Brassica oilseed plants to formulate the sustainable strategies to protect the crop yield under water-limiting conditions. Phytohormones play a key role in fine-tuning various regulatory mechanisms for drought stress adaptation in plants, and the present study explores the response of several physiological stress markers by exogenous supplementation of 24-epibrassinolide (EBL) and jasmonic acid (JA) on two genotypes of Brassica rapa, KS101 and KBS3 under drought stress conditions. The exogenous application of BR and JA, separately or in combination, significantly alleviated the drought stress by improving photosynthetic rate, photosynthetic pigments, stomatal conductance, transpiration rate and antioxidant defence. We observed that concentration of different osmolytes increased and membrane damage significantly reduced by the application of BR and JA. The overall activity of antioxidant enzymes POD, CAT, GR, APX and CAT elevated during all the treatments, be it stress alone or in combination with BR and JA, compared to the control. However, we observed that the BR was much better in mitigating the drought stress compared to JA. Thus, the present study suggests that BR and JA supplementation improves the performance of B. rapa on exposure to drought stress, which hints at the critical role of BR and JA in improving crop productivity in drought-prone areas.

Automated summary

The exogenous application of BR and JA helps alleviate drought stress in Brassica rapa genotypes by modifying osmolyte concentration, levels of antioxidant enzymes, and the photosynthetic system. This study highlights the important role of BR and JA in improving crop productivity in drought-prone areas by fine-tuning various regulatory mechanisms for drought stress adaptation in plants.