Journal
PLANT CELL AND ENVIRONMENT
Volume 44, Issue 9, Pages 3015-3033Publisher
WILEY
DOI: 10.1111/pce.14127
Keywords
BBX; Populus; secondary metabolites
Categories
Funding
- Forest Science Peak Project of College of Forestry, Fujian Agriculture and Forestry University [71201800701]
- JSPS [20K15553]
- Grants-in-Aid for Scientific Research [20K15553] Funding Source: KAKEN
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Through the study of Populus trichocarpa BBX transcription factors, 16 candidate genes were identified, of which 5 were mainly expressed in young leaves and roots, with BBX23 showing a significant response to high light. Overexpression of BBX23 activated the expression of structural genes and MYB transcription factors in the flavonoid pathway, leading to increased accumulation of proanthocyanidins and anthocyanins.
Flavonoids, which modulate plant resistance to various stresses, can be induced by high light. B-box (BBX) transcription factors (TFs) play crucial roles in the transcriptional regulation of flavonoids biosynthesis, but limited information is available on the association of BBX proteins with high light. We present a detailed overview of 45 Populus trichocarpa BBX TFs. Phylogenetic relationships, gene structure, tissuespecific expression patterns and expression profiles were determined under 10 stress or phytohormone treatments to screen candidate BBX proteins associated with the flavonoid pathway. Sixteen candidate genes were identified, of which five were expressed predominantly in young leaves and roots, and BBX23 showed the most distinct response to high light. Overexpression of BBX23 in poplar activated expression of MYB TFs and structural genes in the flavonoid pathway, thereby promoting the accumulation of proanthocyanidins and anthocyanins. CRISPR/Cas9-generated knockout of BBX23 resulted in the opposite trend. Furthermore, the phenotype induced by BBX23 overexpression was enhanced under exposure to high light. BBX23 was capable of binding directly to the promoters of proanthocyanidin- and anthocyanin-specific genes, and its interaction with HY5 enhanced activation activity. We identified novel regulators of flavonoid biosynthesis in poplar, thereby enhancing our general understanding of the transcriptional regulatory mechanisms involved.
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