Journal
PLANT AND CELL PHYSIOLOGY
Volume 62, Issue 12, Pages 1975-1982Publisher
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcab068
Keywords
Arabidopsis plant architecture; Digital archiving; Quantitative evaluation; Three-dimensional reconstruction; Visual volume intersection method
Categories
Funding
- Japan Society for the Promotion of Science KAKENHI [18H05492, 20H03289, 18H05488]
Ask authors/readers for more resources
The study developed a three-dimensional reconstruction system for Arabidopsis plants, allowing researchers to analyze plant morphology at a low cost and with easy adoption.
A plant's architecture contributes to its ability to acquire resources and reduce mechanical load. Arabidopsis thaliana is the most common model plant in molecular biology, and there are several mutants and transgenic lines with modified plant architecture regulation, such as lazyl mutants, which have reversed angles of lateral branches. Although some phenotyping methods have been used in larger agricultural plants, limited suitable methods are available for three-dimensional reconstruction of Arabidopsis, which is smaller and has more uniform surface textures and structures. An inexpensive, easily adopted three-dimensional reconstruction system that can be used for Arabidopsis is needed so that researchers can view and quantify morphological changes over time. We developed a three-dimensional reconstruction system for A. thaliana using the visual volume intersection method, which uses a fixed camera to capture plant images from multiple directions while the plant slowly rotates. We then developed a script to autogenerate stack images from the obtained input movie and visualized the plant architecture by rendering the output stack image using the general bioimage analysis software. We successfully three-dimensionally and time-sequentially scanned wild-type and lazyt mutant A. thaliana plants and measured the angles of the lateral branches. This non-contact, nondestructive method requires no specialized equipment and is space efficient, inexpensive and easily adopted by Arabidopsis researchers. Consequently, this system will promote th reeand four-dimensional phenotyping of this model plant, and it can be used in combination with molecular genetics to further elucidate the molecular mechanisms that regulate Arabidopsis architecture.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available