Gas chromatography-mass spectrometry (GC-MS) profiling reveals substantial metabolome diversity in seabuckthorn (Hippophae rhamnoides L.) berries originating from different geographical regions in the Indian Himalayas

Introduction Seabuckthorn (Hippophae rhamnoides L.) is a high-altitude plant with immense medicinal, nutritional, and therapeutic value. Earlier studies have documented the presence of various useful bioactive substances in this species; however, comprehensive metabolome profiling of seabuckthorn berries originating from different regions of the Indian Himalayas has not been undertaken. Objective Metabolomic profiling of seabuckthorn berries originating from different geographical sites in the Himachal Pradesh and Jammu & Kashmir regions of the Indian Himalayas was performed by using gas chromatography-mass spectrometry. Materials and methods The GC-MS metabolome profiles of seabuckthorn berries collected from different sites (altitude 1,400-4,270 m; average temperature 8 degrees C-27 degrees C) were subjected to multivariate analysis following principal component analysis and hierarchical clustering analysis. Results The GC-MS results showed substantial variability for berry metabolites, including fatty acids, alkyl ethers, and alkyl esters. Fatty acids and their esters were mainly responsible for the variation in the berry metabolome. The metabolite expression profile heat map revealed two distinct groups of seabuckthorn berries originating from Himachal Pradesh (Lahaul and Spiti) and Jammu & Kashmir (Leh, Nubra, and Kargil), the former showing higher expression of metabolites. Interestingly, a strong negative association existed between altitude and the amounts of metabolites such as amides, alkyl esters, alcohols, sugars, and sugar esters. In contrast, temperature showed a strong positive association with ketone and alkyl ether levels. Conclusion GC-MS profiling provides important phytochemical indicators to distinguish between seabuckthorn berries from different geographical sites. Our metabolome profiling analysis generated valuable information that will be useful in the formulation of various seabuckthorn products, benefiting farmers and industries.

Automated summary

Seabuckthorn (Hippophae rhamnoides L.) is a high-altitude plant with significant medicinal and nutritional value. Metabolomic profiling of seabuckthorn berries from different regions of the Indian Himalayas revealed substantial variability in berry metabolites, with fatty acids and their esters playing a major role in the variation. The study also identified distinct groups of seabuckthorn berries from different geographical sites based on metabolite expression, providing important phytochemical indicators for product formulation.