Journal
GASTRIC CANCER
Volume 20, Issue 1, Pages 116-125Publisher
SPRINGER
DOI: 10.1007/s10120-015-0591-z
Keywords
Helicobacter pylori; Gastric cancer; Regulatory T cell; Proliferation; IL-10
Categories
Funding
- Assar Gabrielsson's cancer foundation
- Swedish Cancer Society
- Swedish Research Council
- LUA-ALF-Goteborg
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Background An increase of regulatory T cells, defined as CD25(high)- and/or FOXP3(+)-expressing CD4(+) T cells, within tumors has been reported in several studies. Tregs promote tumor growth by modulating the antitumor immune response, mainly through inhibition of T-cell-mediated tumor cell killing: this has been suggested to be dependent on IL-10 and/or TGF-beta. In stomach cancer, the mechanisms behind the accumulation of Tregs in tumor tissue has not been fully elucidated, and neither has Treg gene expression in situ. Stomach tissue from gastric cancer patients undergoing gastric resection was analyzed using flow cytometry and cell sorting, followed by RT-PCR. We observed that stomach CD4(+) FOXP3(+) T cells proliferated to a higher degree than CD4(+) FOXP3(-) T cells, which may contribute to Treg accumulation in the mucosa. By analyzing DNA methylation, we demonstrated that both proliferating and nonproliferating FOXP3(+) T cells exhibited complete demethylation of the FOXP3 gene, indicating a stable FOXP3 expression in both cell populations. Furthermore, analysis of T-cell populations isolated directly from the tumor and tumor-free mucosa demonstrated that CD4(+) CD25(high) T cells have a higher IL-10/IFN-gamma gene expression ratio but express lower levels of TGF-beta than CD4(+) CD25(low/-) T cells. We demonstrate strong proliferation among regulatory CD4(+) FOXP3(+) CD25(high) T cells in the gastric cancer mucosa. These local Treg express a suppressive cytokine profile characterized by high IL-10 and low TGF-beta and IFN-gamma production.
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