4.4 Article

Evidence for Systemic Reactive Oxygen Species in UVB-mediated Microvesicle Formation

Journal

PHOTOCHEMISTRY AND PHOTOBIOLOGY
Volume 98, Issue 1, Pages 242-247

Publisher

WILEY
DOI: 10.1111/php.13494

Keywords

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Funding

  1. National Institutes of Health [R01 HL062996, R01 ES031087, R01 AI139072, R01 GM137578, R01 GM137394]
  2. U.S. Veteran's Administration Merit Awards [5I01BX000853, BX001792, 1101CX000809]
  3. Senior Research Career Scientist Award [IK6BX004603]

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Recent studies have shown that UVB radiation can generate subcellular microvesicle particles (MVP) both locally and systemically. The ability of UVB to generate MVP is dependent on reactive oxygen species (ROS), and antioxidant vitamins like vitamin C and vitamin E can diminish UVB-induced skin MVPs.
Recent studies have implicated subcellular microvesicle particles (MVP) in the ability of ultraviolet B radiation to exert both local and systemic effects. Indeed, UVB generates MVP (UVB-MVP) in human skin and systemically following phototherapy. The current studies were designed to test the hypothesis that the ability of UVB to generate MVP was dependent upon reactive oxygen species (ROS). To that end, we tested urine samples from subjects undergoing UVB phototherapy for the presence of isoprostanes as well as the oxidized guanosine derivative 8OHdG. We also conducted a clinical study in which volar forearms of subjects were treated with localized UVB and erythema/MVP measured. The same cohort was then treated with 7 days of vitamin C (2 g day(-1)) and vitamin E (1000 IU day(-1)), and UVB-induced MVPs tested on the contralateral forearm. Urine specimens from subjects undergoing phototherapy were found to have increased levels of isoprostanes and 8OHdG, with maximal levels noted 8-16 h post-treatment. Treatment with antioxidant vitamins resulted in diminished UVB-generated skin MVP to baseline levels. These studies suggest that whole-body UVB generates a systemic pro-oxidative response, and that antioxidants can attenuate localized skin UVB-MVPs.

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