Journal
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
Volume 175, Issue -, Pages -Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pestbp.2021.104854
Keywords
Cuticle; Barrier; Chitin; Locust; Insect
Categories
Funding
- National Natural Science Foundation of China [31672364, 31761133021, 32072419, 32070502, 31701794, 31801749]
- German Research Foundation (DFG) [MO1714/101]
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The study revealed the importance of chitin deacetylases CDA1 and CDA2 in the function of cuticle barrier in the migratory locust, affecting fungal infection efficiency and permeability to organophosphorus pesticides. This suggests that the barrier function of the locust cuticle is not only dependent on surface cuticular hydrocarbons but also on an intact procuticle.
Chitin deacetylases including CDA1 and CDA2, containing a chitin deacetylase domain and an LDL domain, have been reported to be essential for cuticle structure differentiation in different insect species. However, it is yet unexplored whether CDA1 and CDA2 activity is needed for the function of the cuticle as a barrier against pathogen and xenobiotics penetration. In this study, we studied the efficiency of fungal infection in the migratory locust Locusta migratoria in dependence of LmCDA1 and LmCDA2 function. Second instar nymphs injected with dsRNA against LmCDA1 and LmCDA2 transcripts were less resistant against the infection by the fungus Metarhizium anisopliae than control nymphs. At the same time, permeability to organophosphorus pesticides was increased in these nymphs. Interestingly, the CHC amounts at the cuticle surface were unaffected upon LmCDA1 and LmCDA2 reduction. These results suggest that the barrier function of the locust cuticle not only depends on surface CHCs, but also on an intact procuticle.
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